A universal fluorescence-based toolkit for real

由 U Sivagnanam 著作 · 2016 · 被引用 8 次 — Nuclease activity: gel assay. Nuclease assays were performed with 20 pmol of purified recombinant hPLSCR1, 200 ng of yeast/ human genomic DNA as ...

2011年4月6日 — Nuclease activity assay stained by EtBr · 1. 若以20% TBE gel分析，EtBr stain染膠30sec~1min，退染distain>20min · 2. anneal complementary pairs of ...

由 C Cappel 著作 · 2021 · 被引用 14 次 — Quantification and characterization of the 5' exonuclease activity of the lysosomal nuclease PLD3 by a novel cell-based assay · Authors · Affiliations.

In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em = 304/369nm). The kit is simple, sensitive, high- ...

由 EC Sheppard 著作 · 2019 · 被引用 12 次 — Conventional nuclease assays predominantly involve the use of radioactive labelling to visualise DNA substrates on an agarose gel. The use of ...

由 P Wyrzykowska 著作 · 2021 — Summary: The nuclease assay summarised in this protocol is a powerful and easy toolkit for analysis of the activity of enzymes connected to DNA/ ...

由 M Fernández-Sierra 著作 · 2015 · 被引用 4 次 — In this work, we present fluorescence-based assays for real-time measurements of exonuclease and endonuclease kinetics. Our assays employ the oxazole yellow ...

To measure nuclease S1 activity, a spectrophotometric rate-determination assay is used at 260 nm. One unit of enzyme will cause 1 μg of single-stranded ...

It is essential that probes used in NPA analysis are all of a discrete length. High specific activity single-stranded RNA probes can be produced by in vitro ...

The initial quality control assay for all components is an analysis for the presence of endo- and exonucleases. This involves incubating the test component with ...